alkyne azdye 647 (catalog no. 1301) Search Results


alkyne azdye 647  (Click Chemistry Tools)
86
Click Chemistry Tools alkyne azdye 647
( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.
Alkyne Azdye 647, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkyne+azdye+647+(catalog+no%2E+1301)/647+alkyne+azdye/pmc12904167-295-60-62
Average 86 stars, based on 1 article reviews
alkyne azdye 647 - by Bioz Stars, 2026-10
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cyanine 3 nhs ester  (Lumiprobe)
95
Lumiprobe cyanine 3 nhs ester
( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.
Cyanine 3 Nhs Ester, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkyne+azdye+647+(catalog+no%2E+1301)/Cyanine3+NHS+ester/ppr0420853-339-106-134
Average 95 stars, based on 1 article reviews
cyanine 3 nhs ester - by Bioz Stars, 2026-10
95/100 stars
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cyanine 5 nhs ester  (Lumiprobe)
97
Lumiprobe cyanine 5 nhs ester
( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.
Cyanine 5 Nhs Ester, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkyne+azdye+647+(catalog+no%2E+1301)/Cyanine5+NHS+ester/ppr0420853-339-117-134
Average 97 stars, based on 1 article reviews
cyanine 5 nhs ester - by Bioz Stars, 2026-10
97/100 stars
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sulfo cyanine 3 nhs ester  (Lumiprobe)
95
Lumiprobe sulfo cyanine 3 nhs ester
( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.
Sulfo Cyanine 3 Nhs Ester, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkyne+azdye+647+(catalog+no%2E+1301)/Sulfo-Cyanine3+NHS+ester/ppr0420853-339-128-134
Average 95 stars, based on 1 article reviews
sulfo cyanine 3 nhs ester - by Bioz Stars, 2026-10
95/100 stars
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version 10.9  (Becton Dickinson)
90
Becton Dickinson version 10.9
( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.
Version 10.9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkyne+azdye+647+(catalog+no%2E+1301)/version+10+9/pm37478011-232-263-264
Average 90 stars, based on 1 article reviews
version 10.9 - by Bioz Stars, 2026-10
90/100 stars
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nk1.1 (mouse anti-mouse, pk136, pe  (Becton Dickinson)
90
Becton Dickinson nk1.1 (mouse anti-mouse, pk136, pe
( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.
Nk1.1 (Mouse Anti Mouse, Pk136, Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkyne+azdye+647+(catalog+no%2E+1301)/propidium+iodide/pm37478011-232-2-11
Average 90 stars, based on 1 article reviews
nk1.1 (mouse anti-mouse, pk136, pe - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.

Journal: Science Advances

Article Title: Mechanical loading induces the longitudinal growth of muscle fibers via a rapamycin-insensitive mechanism

doi: 10.1126/sciadv.aec5134

Figure Lengend Snippet: ( A ) Illustration of how an azide-bearing analog of methionine, called ANL, can get incorporated into NSPs in mice that express a mutated form of the methionyl-tRNA synthetase (MetRS L274G ). Once incorporated, a click reaction with a fluorophore bearing a reactive DBCO or alkyne group can be used to label the azide on the ANL. ( B ) Genotyping (polymerase chain reaction) and Western blot (WB) analysis of tails from the following: (i) floxed STOP GFP-2A-MetRS L274G transgenic mice, (ii) mice that express CMV-Cre plus floxed STOP GFP-2A-MetRS L274G , and (iii) MetRS L274G+/+ mice that ubiquitously coexpress GFP-2A-MetRS L274G in the absence of CMV-Cre. ( C to F ) MetRS L274G+/+ mice were subjected to an MOV or sham (control) surgery. After 7 days, the mice were injected with ANL or the vehicle (PBS), and the plantaris muscles were collected 24 hours later. (C) Representative in-gel fluorescence images showing the DBCO-Cy5.5–labeled NSPs and total protein levels in each sample. (D) Quantification of the NSP to total protein ratio expressed relative to the mean of the control samples. (E) Representative cross section showing Alkyne-AZDye 647–labeled NSPs and total protein from a pair of plantaris muscles (control and MOV) that were visualized with fluorescence microscopy. Scale bar, 500 μm. (F) NSP to total protein ratio for each sample was expressed relative to the mean of the control samples. For illustrative purposes, the signals for Cy5.5 and AZDye 647 were pseudo-colored green. Values in the bars are presented as the group means ± SEM. n = 4 muscles per group. The data were analyzed with a Student’s t test (D) or a paired t test (F). * denotes significantly different from control, P < 0.05.

Article Snippet: Specifically, a 300 μl of reaction mixture (enough for six sections) was prepared by adding the following reagents in the order listed along with vortexing between each addition: (i) 277.5 μl of PBS, (ii) 1.5 μl of 20 mM Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine in DMSO, (iii) 3 μl of 100 mM CuSO4 in diH 2 O, (iv) 18 μl of 50 μM alkyne-AZDye 647 (Click Chemistry Tools #CCT-1301) in DMSO or 6 μl of 500 μM alkyne-AZDye 555 (Click Chemistry Tools #CCT-1289) in DMSO, and (v) 6 μl of 50 mM Tris(2-carboxyethyl)phosphine in diH 2 O.

Techniques: Polymerase Chain Reaction, Western Blot, Transgenic Assay, Control, Injection, Muscles, Fluorescence, Labeling, Microscopy